Separation of four extracellular proteases ofVibrio parahaemolyticusby polyacrylamide gel electrophoresis
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Separation of radiolabelled glycosaminoglycan oligosaccharides by polyacrylamide-gel electrophoresis.
Glycosaminoglycan oligosaccharides generated by treatment of biosynthetically radiolabelled dermatan sulphate and hyaluronic acid with chondroitin AC lyase or testicular hyaluronidase may be resolved into a series of discrete bands by polyacrylamide-gel electrophoresis. Bands were identified by fixation in glacial acetic acid containing 20% (w/v) 2,5-diphenyloxazole followed by fluorography. Th...
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Polyacrylamide gels are typically formed by polymerization of the monomer acrylamide crosslinked to the co-monomer, N,N’-methylenebis-acrylamide, commonly called BIS. This process is a freeradical polymerization that requires an initiator, usually ammonium persulfate, and a catalyst like N,N,N’,N’-tetraethylmethylenediamine (TEMED). A distinct advantage of polyacrylamide gel systems is that by ...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1982
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1982.tb00052.x